Analysis Note

ControlEOMA and BMS2 endothelial cell lysates

Application

Studies of cell-cell interaction

Cell adhesion assays

Inhibition of lymphopoieisis in Whitlock-Witte long-term marrow cultures

Immunoprecipitation of the VCAM-1 integrin complex

Detection of VCAM-1 cells by immunofluorescence and immunohistochemical staining: acetone or fresh frozen sections work best; although zinc formalin or PLP fixations are reported to work but they have not be verified by Chemicon.

Optimal working dilutions must be determined by the end user.

Anti-VCAM-1 Antibody, clone M/K is an antibody against VCAM-1 for use in FC, IH & IP.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Linkage

Replaces: MAB2242

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Format: Purified

Specificity

Recognizes Murine vascular adhesion molecule (VCAM-1), single chain glycoprotein of Mr 100- kDa (under reducing conditions). VCAM-1 is present on the +/+ 2.4 stromal cell clone, large cells in bone marrow, and a subset of thymic macrophages. The stromal cell antigen is also detectable on the EOMA and BMS2 endothelial cell lines. The antibody reacts with all known isoforms of murine VCAM-1.